The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. SpectraMax® iD3/iD5. Microplate Reader. Figure 3. *On the SpectraMax i3x reader only. 9 x 10-15. 19 Switch on the SpectraMax i3x plate reader and launch SoftMax Pro software. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Get a front row view of your cells. Contact Us Phone: +1. We would like to show you a description here but the site won’t allow us. Explore a wealth of applications with the future-ready SpectraMax i3x. The dynamic range of the assay is about 3. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. The SpectraMax i2X is used for cell migration, cell. Spectranet customer portalThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. It allows you to explore cellular pathways and protein activation and expression in one system. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Then, the cell area was calculated for each. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Controls containing all reaction components with either DI . Check software compatibility with Windows 10 and locate your software installer. *On the SpectraMax i3x reader only. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Get Pricing. i3x. 00800 665 32860. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Italic and boldface type are used for emphasis. Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. Sensitive TRF detection of two simple, mix-and-measure assay workflows. 2 in. . 22 μ m fi ltration unit (Sigma, cat. SpectraMax i3x Multi-Mode Detection Platform User Guide. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. Afterward, the absorbance was measured at 450 nm on a Microplate Reader (Spectra Max i3x, Molecular Devices, USA). 2 in. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Molecular Devicesの全商品を見る. Price from $9. SpectraMax i3x and Paradigm readers require the FP-FLUO. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. Ease of use. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. Download PDF. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. , Fisher Scienti fi c, cat. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Ferguson, Ph. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Find regional support. 2. 4 mg mL −1; 1. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 1. Figure 4. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. *On the SpectraMax i3x reader only. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. It allows you to explore cellular . The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. eu@moldev. The SpectraMax ® i3x reader provides the. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . XPS spectra were obtained from PHI Versaprobe II. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. GFP expression imaged on the cytometer. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. . More Product Information. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Prismatic colour and motion for everyone. It also integrates with the. In this product video, see how the SpectraMax M5 quickly reads samples. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. To learn more about the device, please watch this short video. Find regional support. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. each new assay. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Also refer to this Multimode Reader Comparison Chart. User-upgradeable detection modules allow you to expand the capabilities of your microp. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Published Dec 16, 2020 | Updated Dec 16, 2020. This is not due to a temperature-dependent alteration in cellular autofluorescence,. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. StainFree Cell Detection Algorithm. However, no piece of equipment is immune to breaking down. 0´ 104 1. Increased fluorescence performance. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. 13. SpectraMax i3x, onboard luminescence 121. com Check our website for a current listing of worldwide distributors. 313 reference gain check fail. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. 58% for the detection module and 2. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 306 plate air cal fail,low light. As. The treatments were. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Order Now. o For example: Ex: 485/Em 525/Cutoff 515 4. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 4. 吸収度マイクロプレートリーダー SpectraMax® Mini. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Our patent-pending StainFree Cell Detection Algorithm enables cell counting and confluency measurements. SoftMax Pro GxP Compliance Software. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. 4 μM), prepared as described in section 2. Winnersh Triangle. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 305 reference level saturation. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. 314 low lamp level warning. Detection of pro-inflammatory factors. i3. SpectraMax ® iD3 and iD5 Multi-Mode . Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Increased fluorescence performance. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. Accessories for SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. com. Replicate values were averaged and imported into Design-Expert 8. Description 1. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Find regional support. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 635. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 306 plate air cal. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. The SpectraMax i3x reader provides the With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Thetypeof. 04, 0. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. Prime function 20 amol. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax i3x Multi-Mode Detection Platform User Guide. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. MTT was purchased from Sigma-Aldrich (St. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Prepare your i3/i3x instrument for shipment. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. TUNE Tunable Wavelength Detection Cartridge. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Italic and boldface type are used for emphasis. Time-. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Figure 1. 0. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Table 1. Molecular Devices are known for both the reliability and the wide uses of their products. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . This instrument also provides the option of modular upgrades for Western Blotting,. Myeloperoxidase (MPO) Assay. Examples: fiPress carefully to engage,fl fiDo not press down. The standard solutions (0. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. 2 (Molecular Devices, San Jose, CA, USA). Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength*On the SpectraMax i3x reader only. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 01 0. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Apoptosis was measured by the Annexin V-FITC/PI Apoptosis Detection Kit. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. 304 signal level saturation. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 3: The cuvette chamber. SpectraMax Microplate Readers. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. g. 313 reference gain check fail. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Native or oxidized AN (40 μM) and FN (0. Description 1. 200-800 nm using Spectra Max i3X microplate reader. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Accordingly, we calculated the Laurdan generalized polarization (GP) using the formula GP = (I 435 − I 490)/(I 435 +. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. applications, including dual luciferase and ATP assays. Patented user-exchangeable cartridge design expands the system's detection capability making it. The SpectraMax ® i3x. Structured Review. 2. Settings not required by a reader are indicated by ‘---’. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Features. 0001 0. Multi-Mode Reader. 4 / 5. Description 1. infoboxeu@moldev. It allows you to explore cellular pathways and protein activation and expression in one system. A mixture of 6-nitro-1H,3H-benzo[de]isochromene-1,3-dione (486 mg, 2. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Description 1. See SpectraMax® Microplate Reader User Manual Download Page. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. 315 can't find zero order. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. #0200-7009) The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 3: The cuvette chamber. Find regional support. edu) to be added as a trained user in iLab. 304 signal level saturation. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. Sunnyvale, CA, USA) with excitation at 475 nm. . 06, 0. Manufacturers try to design their instruments so they can be. The device can be upgraded to include capabilities for Western blotting, imaging and injection. Supplier Page. Average 98 stars, based on 1 article reviews. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. Microplate Readers SpectraMax. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. *On the SpectraMax i3x reader only. Note: Additional readers with similar performance are listed. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Nuclear Receptor. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. Detection of pro-inflammatory factors. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. 0´ 104 6. If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. +44 118 944 8000. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The SoftMax Pro Software provides integrated instrument control. Use the 3. Introduction. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Recommended settings for SpectraMax iD5 and i3x readers. They were allowed to attach and grow overnight in a 37°C, 5% CO 2. china@moldev. *On the SpectraMax i3x reader only. Download PDF. , 2014; Xiong et al. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). , 2021). Eliminate time-dependent substrate addition steps. 吸収度マイクロプレートリーダー SpectraMax® Mini. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. ATP (“flash” luminescence using . The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. AquaMax Microplate Washer. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 7°C (gradient heating design). Unlock your i3/i3x instrument after shipment. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Figure 1. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Promega. each new lot of plates, to ensure that the center of each well is read. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Increased fluorescence performance.